tag:blogger.com,1999:blog-8317659032924518627.post3741002180215538632..comments2023-10-03T07:38:34.367-04:00Comments on The Evilutionary Biologist: This Week's Citation ClassicJohn Dennehyhttp://www.blogger.com/profile/02522347714772131441noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-8317659032924518627.post-6040441444368810762008-06-19T01:11:00.000-04:002008-06-19T01:11:00.000-04:00Very cool. However, with computers, the line is th...Very cool. However, with computers, the line is that the rest is "a small matter of software". That is, though the solution is provably possible, it's still difficult, perhaps impractical. The trouble is, with software, we have some idea how the computer is going to behave. Biology isn't my primary field, so maybe there are already some techniques that are known. But it is doubtful it's to the point where to imagine it is to do it.Stephenhttps://www.blogger.com/profile/03934169832326108710noreply@blogger.comtag:blogger.com,1999:blog-8317659032924518627.post-27317664872385450492008-06-19T01:10:00.000-04:002008-06-19T01:10:00.000-04:00This comment has been removed by the author.Stephenhttps://www.blogger.com/profile/03934169832326108710noreply@blogger.comtag:blogger.com,1999:blog-8317659032924518627.post-15124766321176552782008-05-24T10:09:00.000-04:002008-05-24T10:09:00.000-04:00Clearly a classic paper. I would suggest that thi...Clearly a classic paper. I would suggest that this technology can be (and should be) supplanted by "in vivo recombination" using <I>Saccharomyces cerevisiae</I> and the awesome power of homologous recombination to generate SDM.<BR/><BR/>Here's an early description of the approach,<BR/>Muhlrad D, Hunter R, Parker R. A rapid method for localized mutagenesis of yeast genes. Yeast. 1992 Feb;8(2):79-82.The Loraxhttps://www.blogger.com/profile/13361004494346338824noreply@blogger.comtag:blogger.com,1999:blog-8317659032924518627.post-51631190387140141122008-05-23T11:57:00.000-04:002008-05-23T11:57:00.000-04:00Clyde Hutchinson "teamed up with [Michael] Smith"A...<EM>Clyde Hutchinson "teamed up with [Michael] Smith"</EM><BR/><BR/>And now Clyde works down the hall from me at the J. Craig Venter Institute's San Diego campus along with another Nobel Smith -- Ham Smith!Jonathan Badgerhttps://www.blogger.com/profile/04921990886076027719noreply@blogger.com